Extended Data Fig. 5: Characterization of Trmt6 conditional knockout mice.
From: tRNA-m1A modification promotes T cell expansion via efficient MYC protein synthesis
a. The schematic diagram of the generation of Trmt6 conditional knockout mice. b. The mRNA level of Trmt6 was analyzed by real-time PCR in Trmt6-KO and WT naïve CD4+ T cells. Error bars represent mean ± s.e.m., n = 3 biologically independent samples from 3 independent experiments. **** P < 0.0001; two-tailed, unpaired t-test. c. The protein level of TRMT6 was quantified by immunoblot in Trmt6-KO and WT naïve CD4+ T cells. Representative data of three independent experiments are shown. d. CD4+ T cell subpopulations from the spleens of 8-week-old Trmt6-KO mice and littermate control mice were analyzed by flow cytometry. The fraction of CD4+ effector memory cells (CD44hiCD62Llo) was quantified. Error bars represent mean ± s.e.m., n = 3 biologically independent animals. * P = 0.0140; two-tailed, unpaired t-test. e. The proliferation of WT naïve CD4+ T cells and Trmt6-KO naïve CD4+ T cells in the presence of anti-CD3 and anti-CD28 antibodies, as measured by CellTrace dilution after 3 days. Error bars represent mean ± s.e.m., n = 4 biologically independent samples. *** P = 0.0001; two-tailed, unpaired t-test.
