Extended Data Fig. 6: Deletion Trmt61a did not affect the TCR/ IL-2 signaling pathway.
From: tRNA-m1A modification promotes T cell expansion via efficient MYC protein synthesis
a. Naïve CD4+ T cells obtained from the spleens of Trmt61a-KO and littermate control mice were stimulated for the indicated minutes with anti-CD3 and anti-CD28 antibodies. Phosphorylated proteins of the TCR signaling pathway were detected by immunoblot. Representative data of two independent experiments are shown. b. Naïve CD4+ T cells obtained from the spleens of Trmt61a-KO and littermate control mice were stimulated for 24 hours with anti-CD3 and anti-CD28 antibodies. The expression of IL-2 was quantified by flow cytometry. Error bars represent mean ± s.e.m., n = 4 biologically independent samples. NS: non-significant; two-tailed, unpaired t-test. c. CD4+ T cells obtained from the spleens of Trmt61a-KO and littermate control mice were stimulated for the indicated times with IL-2. The phosphorylation of STAT5 in the IL-2 signaling pathway was detected by immunoblot. Representative data of two independent experiments are shown. d. Naïve CD4+ T cells were obtained from the spleens of Trmt61a-KO and littermate control mice. The expression of CD127 was quantified by flow cytometry. Error bars represent mean ± s.e.m., n = 3 biologically independent samples. NS: non-significant; two-tailed, unpaired t-test.
