Extended Data Fig. 7: mTOR signaling and MYC pathways.
From: tRNA-m1A modification promotes T cell expansion via efficient MYC protein synthesis
a. Naïve CD4+ T cells obtained from the spleens of Trmt61a-KO and littermate control mice were stimulated for the indicated times with anti-CD3 and anti-CD28 antibodies. Phosphorylated proteins of the mTORC1 signaling pathway were detected by immunoblot. Representative data of four independent experiments are shown. b. Heatmap showing the expression of proteins in activated CD4+ T cells for indicated timepoint, data from the PRIDE database under accession numbers PXD004367. c. Gene-set enrichment analysis showing the down-regulated genes in Trmt61a-KO cells involved in MYC signaling. d. Heatmap showing the expressions of transcripts of the cholesterol biosynthesis and amino acid biosynthesis pathways in activated Trmt61a-KO CD4+ T cells vs. activated WT CD4+ T cells (anti-CD3 and anti-CD28 antibodies for 48 hours). e. Naïve CD4+ T cells obtained from the spleens of Trmt61a-KO and littermate control mice were stimulated for 3 hours with anti-CD3 and anti-CD28 antibodies and then treated by MG132 for 3 hours. The level of MYC protein was quantified by immunoblot. Representative data of two independent experiments are shown.
