Extended Data Fig. 2: Characterization of Trmt61a conditional knockout mice.
From: tRNA-m1A modification promotes T cell expansion via efficient MYC protein synthesis
a. The schematic diagram of the generation of Trmt61a conditional knockout mice. b. The mRNA level of Trmt61a was analyzed by real-time PCR in Trmt61a-KO and WT naïve CD4+ T cells. Error bars represent mean ± s.e.m., n = 3 biologically independent samples from 3 independent experiments. **** P < 0.0001; two-tailed, unpaired t-test. c. tRNA-seq detection of the level of each tRNA in activated Trmt61a-KO CD4+ T cells and WT CD4+ T cells (6 hours). Each dot represents one tRNA. Two biologically independent samples are shown (R1, R2). d. Quantification of the m1A/A ratio in tRNA and mRNA by liquid chromatography-mass spectrometry. Error bars represent mean ± s.e.m., n = 3 biologically independent samples. **** P < 0.0001; NS: non-significant; two-tailed, unpaired t-test. e. The development of T cells in the thymus of 8-week-old Trmt61a-KO mice and littermate control mice. Representative data of six independent experiments are shown. f. The percentage and cell number of T cells (CD3+ TCRβ+) were quantified. Error bars represent mean ± s.e.m., n = 4 biologically independent animals. *** P = 0.0008, * P = 0.0111; two-tailed, unpaired t-test. g. The percentage and cell number of CD4+ or CD8+ T cells in spleen were quantified. * P = 0.0100 (CD4), * P = 0.0385 (CD8), NS: non-significant; two-tailed, unpaired t-test. h. The percentage and cell number of CD4+ or CD8+ T cells in lymph nodes were quantified. *** P = 0.0008, ** P = 0.0046, NS: non-significant; two-tailed, unpaired t-test. i. The fractions of effector memory cells (CD44hiCD62Llo) and naïve cells (CD44loCD62Lhi) in spleen were quantified. ** P = 0.0069, * P = 0.0356; two-tailed, unpaired t-test. j. The fractions of effector memory cells (CD44hiCD62Llo) and naïve cells (CD44loCD62Lhi) in lymph nodes were quantified. **** P < 0.0001, *** P = 0.0008, * P = 0.0732; two-tailed, unpaired t-test. Boxes show median, upper and lower quartiles, whiskers show 1.5× IQR on either side (g-j). n = 4 biologically independent animals (g-j).
