Extended Data Fig. 2: Leukocyte dynamics in HSC-T and PDAC patients.
From: Cellular and transcriptional dynamics of human neutrophils at steady state and upon stress
a. Expression of the indicated markers in NDNs and LDNs from controls (n > 10) or G-CSF-treated donors (n > 12). b, c. Histogram (b) and cumulative histogram (c) plot showing the expression levels of CD49d in neutrophil precursors, immature and mature neutrophils. d, e. Contour (d) and cumulative histogram (e) plots showing percentages of EdU+ cells within NDNs and LDNs from BM, CB and PB samples of the indicated patients (BM n = 3, CB n = 6, G-CSF n = 8, HSC-T 2° f.u. n = 3). f, g. Representative histogram (f) and cumulative histogram (g) plot showing expression of the indicated markers in in NDNs and LDNs from controls (n > 11) or G-CSF-treated donors (n > 11). h. Representation of leukocytes dynamics in HSC-T patients. i. Quantification of white blood cells (WBCs), neutrophil, monocyte and lymphocyte count in HSC-T patients. Gray intervals highlight normal ranges. Arrows indicate the beginning of myeloablative conditioning; day 0 indicates the day of HSC-T. j. Counts of monocytes or lymphocytes in PB of controls (n = 8) or HSC-T patients (1° f.u. n = 8, 2° f.u. n = 9, 3° f.u. n = 3). k,l. Percentage of neutrophil precursors, immature and mature neutrophils within PBMCs (k) or LDNs (l) in controls (n = 8) and HSC-T patients (1° f.u. n = 7, 2° f.u. n = 8). m-o. Absolute counts (m) and frequencies of HSPC subsets (n, o) in whole PB (n = 15) or BM (n = 14) of controls and PB of PDAC patients (n = 8). p. Leukocyte counts in whole PB of controls (n = 15) and PDAC patients (n = 8). q. Neutrophil-to-lymphocyte ratio (NLR) in whole PB of controls (n = 15) and PDAC (n = 8) patients. NLR is calculated as the ratio between absolute counts (FACS) of neutrophils and total lymphocytes. r. Leukocyte counts (hemocytometer) and corresponding NLR values in whole PB of controls (n = 10), IPMN (n = 12) and PDAC (n = 15) patients. s. Contour plots showing CD16 and CD11b expression in LDNs of three PDAC patients. t, u. Percentage of neutrophil precursors, immature and mature neutrophils within LDNs (t) and PBMCs (u) of controls (n = 12), IPMN (n = 12) and PDAC (n = 18) patients. v. Percentage of EdU+ cells within neutrophil precursors, immature and mature neutrophils in PB of PDAC patients (n = 6). Gating strategies for the indicated cell types are reported in Supplementary Table 7. Bar plots report data as mean ± SD. Statistical analyses. a, c, g, j, k and r: Kruskal-Wallis test plus two-sided Dunn’s multiple comparison. m, o-q: two-sided Mann-Whitney test.
