Extended Data Fig. 5: NUDT21 directly regulates CD19 mRNA 3′ UTR length. | Nature Immunology

Extended Data Fig. 5: NUDT21 directly regulates CD19 mRNA 3′ UTR length.

From: NUDT21 limits CD19 levels through alternative mRNA polyadenylation in B cell acute lymphoblastic leukemia

Extended Data Fig. 5

(a) CD19 scores for individual sgRNAs spanning the NUDT21 locus in NALM6 domain screen. (b) eCLIP read tracks spanning the CD19 locus in Reh, 697 and TMD8 cells. PureCLIP significant peak signals shown. (c) Pie-chart highlighting genomic distribution of eCLIP peaks shared by BCP-ALL cell lines. (d) MACE-seq reads for NALM6 and (e) log fold-change read counts of the terminal coding exon 14 and 3-UTR junction (intron removed) in 697 and Reh cells comparing sgNUDT21#1 to sgROSA. (f) Sashimi plot of exon-exon junctions across the CD19 locus in Reh, 697 and NALM6 cells. Bulk RNA-seq experiment performed in technical duplicate.

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