Extended Data Fig. 2: ZNF143 ablation alters CD19 mRNA expression independent on chromatin looping. | Nature Immunology

Extended Data Fig. 2: ZNF143 ablation alters CD19 mRNA expression independent on chromatin looping.

From: NUDT21 limits CD19 levels through alternative mRNA polyadenylation in B cell acute lymphoblastic leukemia

Extended Data Fig. 2

(a) Immunoblot analysis of ZNF143 and Actin in NALM6 cells expressing sgROSA or sgZNF143#1 for seven days. (b-c) Protein sizes indicated (b) CD19 scores for individual sgRNAs spanning the CD19 and (c) CD81 locus in NALM6 domain screen. (d) Volcano plots of intra-TAD activity comparing sgROSA and sgZNF143 expressing cell line, Reh, 697 and NALM6 (two-sided t-test followed by false discovery rate (FDR) correction. FDR < 0.01 cutoff). (e) Virtual 4C analysis of CD19 promoter viewpoint generated from Hi-C data of Reh, 697 and NALM6 expressing sgROSA and sgZNF143.

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