Extended Data Fig. 2: Genome-scale screens: quality control metrics.
From: In vivo CRISPR screens reveal the landscape of immune evasion pathways across cancer
a, In vitro and in vivo library recovery. Dotted lines represent abundance at 5th and 95th percentile for each library. b, Replicate autocorrelation analysis. Pearson’s correlations are calculated for the library distribution in one 10-tumor replicate versus any other replicate, two averaged replicates versus any other two, and so on. The mean of all possible combinations is plotted. c, Effect size model for sgRNAs. A natural cubic spline (solid line) was fit between NSG and WT or ICB sgRNA abundances, and the effect size is calculated as the residual from the spline. d, Normalized effect size distributions for the WT versus NSG and ICB versus NSG comparisons; distributions for control sgRNAs are shown in dotted lines, distributions for gene-targeting sgRNAs are shown in solid lines.
