Extended Data Fig. 8: Conditional loss of Bcl-xL impairs survival of migratory, but not resident, cDC1.

a, Representative histogram of Bcl-xL staining in cDC2 from mesenteric lymph nodes of WT and BclxL^cKO mice injected i.p. with PBS (black) or agonistic CD40 antibody (red, blue). b, Representative histograms of CD40 and MHC-II surface expression in migratory cDC1 of TDLN from tumor-bearing WT (Xcr1^+/+ Bclx^fl/fl), Cd40^cKO (Xcr1^Cre/+ Cd40^fl/fl), and BclxL^cKO (Xcr1^Cre/+ Bclx^fl/fl) mice. c-d, Quantification of migratory cDC1 as a percentage (c) and number (d) from TDLNs of day 6 tumor-bearing BclxL^WT, BclxL^cKO, Cd40^WT, Cd40^cKO, and Ptgs2^cKO (Xcr1^Cre/+ Ptgs2^fl/fl) mice. Data represent pooled biologically independent samples from two independent experiments (n = 4-5 for BclxL^WT, n = 8 for BclxL^cKO, n = 5 for Cd40^WT, n = 4 for Cd40^cKO, and n = 2 for Ptgs2^cKO). Data are represented as mean values +/− s.d. *P = 0.0249, 0.0240; ***P = 0.0001; *P = 0.0257; ns, not significant. e, Representative flow plots of resident cDC1s (red boxes) and cDC2s (black boxes) in TDLNs of day 6 1956-mOVA-bearing BclxL^WT, BclxL^cKO, Cd40^WT, Cd40c^KO, and Ptgs2^cKO mice. Cells are pregated as B220⁻ CD326⁻MHC-II⁺ CD11c^hi. Numbers are percentages of cells in the indicated gates. Data represent pooled biologically independent samples from two independent experiments. f-g, Quantification of resident cDC1s in e as a percentage (f) and number (g) from TDLNs of day 6 tumor-bearing BclxL^WT, BclxL^cKO, Cd40^WT, Cd40^cKO, and Ptgs2^cKO mice. Data represent pooled biologically independent samples from two independent experiments (n = 4-5 for BclxL^WT, n = 8 for BclxL^cKO, n = 5 for Cd40^WT, n = 4 for Cd40^cKO, and n = 2 for Ptgs2^cKO). Data are represented as mean values +/− s.d. ns, not significant. c,d,f,g: Brown–Forsythe and Welch ANOVA with Dunnett’s T3 multiple comparisons test.

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