Extended Data Fig. 3: Susd2^−/− CD8⁺ cells exhibit increased antitumor effector function.

a, Flow cytometry analysis of IFN-γ⁺CD8⁺ T cells, GzmB⁺CD8⁺ T cells and TNF⁺CD8⁺ T cells in different OVA_257–264 dosage stimulated splenocytes isolated from WT or Susd2^−/− OT-I mice. b-e, CD8⁺ T cells were isolated from total splenocytes of either WT or Susd2^−/− OT-I mice left untreated or stimulated with OVA_257–264 for 3 days and were subjected to RNA-seq assay. The volcano plot of RNA-seq data demonstrates differential gene expression between WT and Susd2^−/− CD8⁺ T cells at Day 0 (b) and Day 3 (d). A heat map of the top thirty genes representing genes differentially expressed between WT and Susd2^−/− CD8⁺ T cells at Day 0 (c) and Day 3 (e). f, Intracellular accumulation of IFN-γ in CD8⁺ T cells isolated from WT or Susd2^−/− OT-I mice that were co-cultured with either WT or Susd2^−/− bone marrow-derived dendritic cells (BMDCs) that have been pulsed with OVA_257–264. a,f, n = 3, b-e, n = 4. n, number of mice per group. a,f, data are representative of four independent experiments. b-e, data are representative of two independent experiments. b,d statistical significance was calculated using two-sided Wilcoxon’s rank-sum test and adjusted with Bonferroni’s correction. Statistical significance was determined by two-way ANOVA followed by Sidak’s multiple comparisons test(a,f) with P values noted in the figure. All data are mean ± SD.

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