Extended Data Fig. 4: Susd2 deficiency does not affect effector function of CD4+ T cells or inhibitory function of Treg cells.
From: SUSD2 suppresses CD8+ T cell antitumor immunity by targeting IL-2 receptor signaling
a, Flow cytometry analysis of IFN-γ+CD4+ T cells, GzmB+CD4+ T cells and TNF+CD4+ T cells in OVA323–339 stimulated splenocytes isolated from WT or Susd2−/− OT-II mice. b, Flow cytometry analysis of intranuclear level of Foxp3 in spleen CD4+ T cells from WT or Susd2−/− mice. c, Cell proliferation of naïve CD4+ T cells upon stimulation with CD3-CD28 antibody in the absence or presence of WT or Susd2−/− Treg cells at the indicated cell: cell ratio was measured by the staining of carboxyfluorescein diacetate succinimidyl ester (CFSE), followed by FACS analysis. a–c, n = 3. n, number of mice per group. Data are representative of four independent experiments. Statistical significance was determined by two-tailed, unpaired Student’s t-test, there is no significant difference between WT and Susd2−/− group in a–c (P > 0.05). All data are mean ± SD.
