Extended Data Fig. 7: Identification of EL4-hCD19 cells and CAR T cells.
From: SUSD2 suppresses CD8+ T cell antitumor immunity by targeting IL-2 receptor signaling
a, Validation of EL4 thymoma cell line expressing human CD19 (EL4-hCD19) with the deletion of its intracellular domain. b, Percentages of CD8+ T cells retrovirally transduced with a chimeric antigen receptor (CAR) containing a portion of hCD19 single chain variable fragment (ScFv) fused with signaling domains of mouse CD28 and mouse CD3ζ sequence (with first and third ITAMs of the CD3ζ molecule inactivated) before and after cell sorting were assessed by the staining with anti-Thy1.1 antibody. c, Transcript of Susd2 in CAR T cells that have been electroporated with scrambled gRNA(sgRNA) or Susd2 gRNA-Cas9 nucleoprotein (RNP) complex, sgRNA versus Susd2 gRNA (P = 0.0010). c, n = 3. a–c, data are representative of two independent experiments. Statistical significance was determined by two-tailed, unpaired Student’s t-test (c) with P values noted in the figure. The data represent mean ± SD.
