Extended Data Fig. 2: Susd2 deficiency does not affect intratumoral myeloid cells, NK cells and CD4⁺ T cells.

a-g, Flow cytometry analysis of CD11b⁺F4/80⁺ macrophages (a), CD11b⁺CD11c⁺ dendritic cells (b), CD11b⁺Ly6C⁺ monocytes (c), CD11b⁺Ly6G⁺ neutrophils (d), NK1.1⁺ NK cells (e), IFN-γ⁺NK1.1⁺ NK cells (f), IFN-γ⁺CD4⁺, GzmB⁺CD4⁺ and TNF⁺CD4⁺ T cells (g) in MC38 tumor isolated from WT or Susd2^−/− mice at Day 18. h,i, Spectral flow cytometry analysis of intratumor CD8⁺ T cells from WT and Susd2^−/− mice at day18 post MC38 tumor inoculation. UMAP of individual marker expression patterns (h) and frequencies of individual clusters of WT and Susd2^−/− samples (i), Boxes represent median and 25th to 75th percentiles, whiskers are minimum to maximum values excluding outliers (two-sided Wilcoxon’s rank-sum P value). j-l, Flow cytometry analysis of PD-1⁺CD8⁺ T cells and LAG-3⁺CD8⁺ T cells in MC38 (j), EG7 (k) or B16-OVA (l) tumors isolated from WT or Susd2^−/− mice at day 18 post tumor inoculation. a–d,g,j-l, n = 5, e,f,i, n = 8. n, number of mice per group. a–d,g,j,k,l, data are representative of three independent experiments, e,f,i, data are representative of two independent experiments. a-g, j-l, statistical significance was determined by two-tailed, unpaired Student’s t-test, there is no significant difference between WT and Susd2^−/− group (P > 0.05). All data are mean ± SD.

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