Extended Data Fig. 3: Impact of pre-existing antibody levels on transcriptional responses to influenza vaccination.

a) Differentially expressed modules at Day 1 (FDR < 0.05, t-test between mean fold changes) between participants with high and low baseline antibody titers (negative score indicates increased expression in the low baseline antibody group). Differentially expressed modules at Day 7 between high and low baseline antibody groups. c, d) Boxplots of (C) IFN signature module M75 expression at Day 1 and (D) plasma cell module M156.1 expression at Day 7 between high and low baseline antibody groups at Day 1. Center line, median; box limits, upper and lower quartiles; whiskers, 1.5x interquartile range. Day 1: SDY1276_D: High – n = 35, Low – n = 31; SDY1276_V: High – n = 31, Low – n = 31; SDY180: High – n = 4, Low – n = 4; SDY56: High – n = 4, Low – n = 7; SDY80: High – n = 14, Low – n = 14. Day 7: SDY1119: High – n = 6, Low – n = 6; SDY180: High – n = 4, Low – n = 4; SDY270: High – n = 10, Low – n = 9; SDY56: High – n = 4, Low – n = 7; SDY61: High – n = 3, Low – n = 3; SDY63: High – n = 3, Low – n = 4; SDY640: High – n = 6, Low – n = 4; SDY80: High – n = 13, Low – n = 13. e, f) Line graphs of (E) M75 and (F) M156.1 expression across time in high and low baseline antibody groups. Error bars represent standard error of the mean. Day 1: High – n = 88, Low – n = 87; Day 3: High – n = 83, Low – n = 88; Day 7: High – n = 49, Low – n = 50; Day 14: High – n = 64, Low – n = 66; In C-F, statistical differences were determined via two-sided unpaired Student’s t-tests. *p < 0.05, **p < 0.01, ***p < 0.001.