Fig. 2: Participants have distinct pre-vaccination transcriptomic profiles.
From: Pan-vaccine analysis reveals innate immune endotypes predictive of antibody responses to vaccination
Hierarchical clustering (Euclidean distance metric and complete linkage agglomeration method) of pre-vaccination samples (day −7 and day 0) based on the expression of the BTMs and hallmark gene sets. The overall transcriptomic activity of gene sets/modules was estimated using sample-level enrichment analysis (SLEA). Three groups of participants/endotypes can be identified by cutting the dendrogram. Average SLEA score of the four hallmark inflammatory gene sets (bold row labels; inflam.gs), discretized in tertiles, is shown as sample annotation. Endotypes were designated as high (inflam.hi), low (inflam.lo) and middle (inflam.mid) inflammatory pathways. For each of the seven supersets of hallmark and BTM gene sets, ten canonical genes annotated to NK cells, T cells, B cells, E2F/MYC, inflammation, monocytes/DCs and ISGs, respectively (heat map). TH2, type 2 helper T cell.
