Extended Data Fig. 5: Myeloid cells and astrocytes in the hippocampus of Mr1−/− mice.
a, UMAP analysis for the entire population of live cells isolated from the hippocampus of 7-month-old Mr1+/+ and Mr1−/− mice. b, Representative flow cytometry profiles for monocytes and neutrophils in the hippocampus of 7-month-old Mr1+/+ and Mr1−/− mice. Lungs from wild-type mice were used as a positive control for gating monocytes and neutrophils. c, Numbers of monocytes and neutrophils in the hippocampus of 7-month-old Mr1+/+ and Mr1−/− mice. d, Representative profile of immunofluorescence staining of GFAP in the HP DG region of 7-month-old Mr1+/+ and Mr1−/− mice. e, Numbers of astrocytes (GFAP+ cells) in the HP DG regions. f, UMAP analysis of astrocytes in the hippocampus of 7-month-old Mr1+/+ and Mr1−/− mice. scRNA-seq was performed with FACS-sorted live cells in the hippocampus. Gating strategy to sort live cells for scRNA-seq is shown in Supplementary Fig. 2. UMAP profiles for total live cells in the hippocampus are provide in a. The data subset for microglia was created using the ‘subset’ function of Seurat. g, Feature plots depict expression of Il33 and Gfap in astrocytes. h, Genes highly expressed by each astrocyte subset. i. Proportions of each astrocyte subset in Mr1+/+ and Mr1−/− mice. j, Differentially expressed genes (DEG) in the total astrocyte population and in each astrocyte subset, between Mr1+/+ and Mr1−/− mice. k, Feature plots depict expression of Uba52 in astrocytes. Error bars = Mean ± SE. Data are from 6 mice per group, pooled from two independent experiments (b-e), or are from 6 mice pooled per group (a, f-k). *P < 0.05, **P < 0.01, n.s = not statistically significant (P > 0.05) using two-sided Student’s t-test (c, e), or two-sided Wilcoxon rank sum test (k); exact P values are provided in the source data (c, e) or Supplementary Table 11.
