Extended Data Fig. 4: Probing the extent of ex-vivo Microglia-T cell doublet formation. | Nature Immunology

Extended Data Fig. 4: Probing the extent of ex-vivo Microglia-T cell doublet formation.

From: Cognate microglia–T cell interactions shape the functional regulatory T cell pool in experimental autoimmune encephalomyelitis pathology

Extended Data Fig. 4

(a) Scheme describing the mixing experiment where brains from a whole body GFP or whole body TdTomato mice were mixed together or isolated separately. Mixed brains went through enzymatic digestion and cell isolation process together. Gating strategy showing the extent of GFP/TdTomato cells from each population. (b) Calculation of the probability to obtain spurious vs. real doublets, showing that most of the doublets are not spurious (with kind help of Shalev Itzkovitz, WIS).

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