Extended Data Fig. 4: Development of DP thymocytes in Lck variant mice.
From: Unique roles of co-receptor-bound LCK in helper and cytotoxic T cells
(a-b) Thymocytes from Lck variant mice were analyzed by flow cytometry. (a) Representative histograms are shown. (b) Normalized expression of indicated surface markers in the Lck variant mice (average gMFI in LckWT/WT mice was set as 1). Individual values and medians are shown. LckWT/WT: 25 mice in 11 independent experiments, LckKO/KO: 13/7, LckKR/KR: 12/6, LckCA/CA:18/8, LckCA/KR: 11/5. The statistical significance was calculated by Wilcoxon Signed Rank Test (vs the value of 1). (c) Additional 4 independent experiments described in Fig. 2a-b. (d) Representative mice from Experiment 3 described in Fig. 2a-b. (e-h) Basal phosphorylation of TCRζ (e-f) and ZAP70 (g-h) in the indicated thymocyte subpopulations (TCRβlow DP, TCRβhigh DP, TCRβhigh SP8, TCRβhigh SP4) in the indicated Lck variant mouse strains was determined by flow cytometry. (e,g) Overall data are shown. Data normalized to gMFI of LckWT/WT mice are displayed. n = 5 independent experiments/mice. The statistical significance was tested using one sample t-test values. (f, h) Representative experiments out of 5 in total.
