Extended Data Fig. 7: Analysis of T-cell compartment in anti-viral and anti-tumor responses.

(a-g) Indicated Lck variant mice were infected with LCMV and the splenocytes were analyzed on day 8 post-infection. n = 8 (Lck^CA/KR) or 9 (other strains) mice in 2 (Lck^CA/CA) or 3 (other strains) independent experiments. Individual mice and means are shown. The statistical significance was calculated using a Mann-Whitney test. Related to Fig. 3a-e. (a) Counts of CD8⁺ cells in the spleens. (b) Counts of CD4⁺ cells in the spleens. (c) Counts of GP33 4mer⁺ and NP396 4mer⁺ CD8⁺ cells in the spleens. (d) Frequencies of CD44⁺ CD49d⁺ cells from NP396 4mer⁺ CD8⁺ cells in the spleens. (e) Frequencies of KLRG1⁺ CD127⁻ cells from NP396 4mer⁺ CD8⁺ cells in the spleens. (f) Frequencies of T_FH cells expressing FOXP3 and counts of FOXP3⁻ T_FH cells in the spleens. (g) Frequencies and counts of GP66 4mer⁺ CD4⁺ cells in the spleens. (h-j) Tumor-infiltrating CD8⁺ T cells were isolated from MC-38-OVA tumors from Lck variant mice and analyzed by flow cytometry. n = 9 for Lck^WT/WT, Lck^CA/KR, n = 8 for Lck^CA/CA, Lck^KR/KR from 3 independent experiments. (j) Counts of tumor-infiltrating CD8⁺ T cells. Individual mice and medians are shown. (k) An example of gating strategy for the detection of K^b-OVA 4mer⁺ cells. (i) Frequencies and counts of K^b-OVA 4mer⁺ cells in tumors. Individual mice and medians are shown.

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