Extended Data Fig. 6: cTFH cell populations are expanded post-COVID-19 in PBMC.
From: Adaptive immune responses to SARS-CoV-2 persist in the pharyngeal lymphoid tissue of children
a,b. UMAP of unsupervised clustering of surface markers from flow cytometric analysis of CD4+ T cells from PBMCs (a) with heatmaps of marker/antibody expression overlayed (b). c. Intracellular cytokine and cytotoxic factor production by various circulating TFH cell (cTFH) subsets in PBMC gated by CXCR3 and CCR6 from post-COV donors (n = 4) following PMA/ionomycin stimulation. Mean cell frequency shown in heat map. d. Frequency of CD45RA+CCR7+CD28+CD27+CD95+ CD4+ T stem cell-like memory (TSCM) cells in PBMC of post-COV (n = 16) and UC (n = 41), p = 0.007. Each symbol represents one donor. Means ± S.D. displayed on bar plots. Significance calculated with two-sided Mann–Whitney U-test. **p < 0.01.
