Extended Data Fig. 5: Related to Fig. 3c: Relationship between differential accessibility of regulatory regions and transcriptome.

a, Differential peaks between young and older individuals at indicated TCR stimulation conditions and time points are plotted as logFC versus log mean reads per peak. Comparisons were done by robust empirical Bayes moderated statistical test; a Benjamini–Hochberg adjusted P value of 0.05 was used as cutoff. b, Aggregate genome accessibility tracks (top panel) of representative sites of cluster 6–10 shown in the heat plot in Fig. 3c. Cyan-shaded areas indicate peaks that are more open in young compared to older adults, magenta-shaded areas are more open in old. Transcript data from corresponding genes as determined by RNA-seq (lower panel) and shown as mean + s.e.m. n = 8 biologically independent samples. c, LogFC difference of transcripts differentially expressed between young and older adults from 48-h RNA-seq data is plotted against logFC difference of differential peaks from ATAC-seq data annotated to the same gene at the indicated time point. Data are fitted with two-sided simple linear regression. P < 0.0001.