Extended Data Fig. 6: Related to Fig. 3h: Time course of TF expression after STAT5 inhibition.
Naive CD4+ T cells from older adults were activated for 0–24 h with polystyrene beads coated with 1 µg mL-1 anti-CD3 and anti-CD28 in the presence of DMSO solvent control or a STAT5 inhibitor. Representative western blots (top panel). Summary data from 3 experiments showing band intensities of IRF4 and BATF relative to β-actin (bottom panel) and expressed as mean + s.e.m. n = 3 biologically independent samples. Data were analyzed with two-sided paired t-test. *P < 0.05 (p = 0.02), **P < 0.01 (p = 0.0094).
