Extended Data Fig. 7: Related to Fig. 4: Single cell epigenetic and transcriptional analysis of naive CD4+ T cell responses. | Nature Immunology

Extended Data Fig. 7: Related to Fig. 4: Single cell epigenetic and transcriptional analysis of naive CD4+ T cell responses.

From: Aging-associated HELIOS deficiency in naive CD4+ T cells alters chromatin remodeling and promotes effector cell responses

Extended Data Fig. 7

a, CD31 and PTK7 surface expression on naive CD4+ T cells. b, Nuclei pooled from unstimulated and activated naive CD4+ T cells were subjected to scATAC- and scRNA-seq. Imputed CD69 gene expression is projected on the UMAP of scATAC-seq. Clusters of resting and activated cells were distinguished based on containing CD69-expressing cells (left panel). UMAP maps of scATAC-seq and scRNA-seq data are shown for activated cells (right panels). c, B3GAT1 and KLRG1 gene expression were projected onto scMultiome UMAPs (top panel). Genome tracks of bulk ATAC-seq and gene expression in bulk RNA-seq expressed as mean + s.e.m. (bottom). n = 8 biologically independent samples. d, IL4 and IL17A gene expression were projected onto scMultiome UMAPs (top panel). Genome tracks of bulk ATAC-seq at 24 h of TCR stimulation (bottom).

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