Extended Data Fig. 1: Related to Fig. 1: Titration of TCR stimulation.
a, Naive CD4+ T cells were stimulated with polystyrene beads coated with indicated amounts of anti-CD3 Ab together with a constant amount of 1 µg mL-1 antiCD28. Bead-cell conjugates were gated and the percent of bound to total cells were determined. b, Histograms of phosphorylated ERK gated on bead-bound cells at 0 and 5 min stimulated with indicated amounts of anti-CD3. c, Mean fluorescence intensity (MFI) of phosphorylated ZAP70, SLP76 and ERK was measured gated on bead-bound cells. d, Histogram of CD69 gated on bead-bound cells at 24 h of stimulation (left panel). Frequency of CD69+ cells as percentage of total bead-bound cells from 0–48 h with indicated anti-CD3 concentrations (right panel). e, Cell Trace Violet dye dilution of naive CD4+ T cells stimulated with 1 µg mL-1 anti-CD3. Experiments shown are representative of three experiments.
