Extended Data Fig. 4: Genome-wide transcriptomic analysis of av-deficient TH cells. | Nature Immunology

Extended Data Fig. 4: Genome-wide transcriptomic analysis of av-deficient TH cells.

From: An αvβ3 integrin checkpoint is critical for efficient TH2 cell cytokine polarization and potentiation of antigen-specific immunity

Extended Data Fig. 4

Extended Data Fig. 4(a) Principal component analysis of the transcriptomes of control versus αv-deficient TH cells. (b) Volcano plot depiction of differentially expressed genes in control versus αv-deficient TH2 cells. (c) & (d) RNA expression of type 2 genes by control or αv-deficient TH cells; Il13 ***P = 0.0002, **P = 0.0012; Il5 ***P = 0.0006, **P = 0.0025; Il4 **P = 0.0046, *P = 0.0167, Gata3 ****P < 0.0001. (e) RNA expression of type-1 signalling genes by control or αv-deficient TH cells; Stat1 ****P < 0.0001, *P = 0.0438; Isg20 *P = 0.0148; Runx3 **P = 0.0052, *P = 0.0220. (c) - (e) mean ± SEM; one-way ANOVA with Tukey’s post-hoc test. (f) Flow cytometric analysis of Runx3 expression by αv-deficient TH cells. Data are representative of 2 independent experiments; unpaired two-sided t-test; ***P = 0.0003. (g) KEGG pathway analysis of genes downregulated in αv-deficient versus control TH2 cells.

Back to article page