Fig. 1: A distinct subset of human TH17 cells can express IL-1α.
From: Human TH17 cells engage gasdermin E pores to release IL-1α on NLRP3 inflammasome activation
a, ScRNA-seq and Leiden clustering of human TH17 cells after 5 d of stimulation with anti-CD3 and anti-CD28 monoclonal antibodies. b, IL1A expression in TH17 cells visualized in UMAP. c, Intracellular cytokine staining and flow cytometry of T cell clones generated from TH17 cells that were isolated ex vivo according to their differential expression of chemokine receptors. Left, representative flow cytometric analysis of one TH17 cell clone. Right, cumulative data from the blood of three healthy donors. d, DiVenn plot of DEGs obtained from IL1A+ versus IL1A– human TH17 cells stimulated as described in a (shown as left green circle) and compared with IL1A+ versus IL1A– human LPS-stimulated monocytes (GEO, accession no. GSE159113) (shown as right green circle). Upregulated genes (red circles) and downregulated genes (blue circles) are connected via a gray line to either green circle, indicating its dataset of origin. Gray lines connecting both green circles depict common DEGs between both datasets.
