Extended Data Fig. 5: IL-1α is secreted by an unconventional pathway in human Th17 cells and is not expressed on the cell surface.

a, Th17 cells were stimulated for 5 days with anti-CD3 and anti-CD28 mAbs (48 h plate-bound) before intracellular cytokine staining and flow cytometric analysis on day 5 following PMA and ionomycin restimulation. Representative experiment. b, Cumulative data, Two-sided paired t-test. Each circle indicates an individual healthy blood donor (n = 3 examined over 2 independent experiments). Data are presented as mean ± SEM. c, ELISA of 24 h cumulative supernatants from day 4 to day 5 in the presence or absence of BFA. n.s., not significant. Two-sided paired t-test. Each circle indicates an individual healthy blood donor (n = 4 biologically independent samples examined over 2 independent experiments). Data are presented as mean ± SEM. d, Th17 cells were stimulated and analyzed as in (a). Monocytes were stimulated in the presence and absence of LPS (24 h) and nigericin (last 30 min) and analyzed by intracellular and surface staining and flow cytometry. Representative experiment (n = 3 biologically independent samples).