Extended Data Fig. 8: The GSDME promoter and intronic enhancer region display consensus binding sites for TCR-induced transcription factors as well as for Th17 master regulators.
From: Human TH17 cells engage gasdermin E pores to release IL-1α on NLRP3 inflammasome activation
a, Locations of consensus binding sites of TCR-induced (NFATC-family, NF-κB-family, AP1-family and BATF) and TGFβ-induced (SMAD 2,3,4) transcription factors as well as the Th17 transcription factors RORα and BATF in the regulatory region of GSDME (chr7:24,754,387-24,758,496). The regulatory region and the consensus binding sites were defined and annotated as in Extended Data Fig.6. b, The consensus binding motives were aligned with predicted binding sites in the GSDME promotor/enhancer region. The binding sites with the highest score for each family are indicated. NFATC3-GSDME.1 (chr7:24756897-24756905), RELA-GSDME.1 (chr7:24757878-24757887), FOS::JUN (chr7:24755766-24755775), BATF-GSDME.1 (chr7:24755766-24755776), SMAD4 (chr7:24754928-24754935), RORα (chr7:24758120-24758133).
