Extended Data Fig. 7: Expression of transferrin and other iron metabolism-associated genes in different subsets of skin T cells and splenic iNKT cells.
a, Expression of iron-associated genes from qRT-PCR analysis of immune cells sorted from the skin of WT mice (n = 4 per group). Relative expression was normalized to expression of Gapdh. b, Expression of iron-associated genes from qRT-PCR analysis of skin iNKT, splenic iNKT, and splenic NK1.1-CD4−CD8− (DN) iNKT cells from 3-week-old mice (n = 4 per group). Relative expression was normalized to expression of Gapdh. c,d, Histograms (left) and MFI quantification (right) of Trf (c) and CD71 (d) in whole and NK1.1- DN iNKT cells from the spleen of 3-week-old mice (n = 4). One dot represents one mouse. Significance was determined by paired two-sided Student’s t-test. NS, not significant.
