Extended Data Fig. 8: Defects of iNKT cells in the skin but not spleens or thymi of Trf hpx/hpx mice.
a, b, Comparison of frequency of iNKT (CD3+CD1d-tet+) and Treg (CD3+FOXP3+) cells in spleens (a) and thymi (b) of Trf+/hpx (d6: n = 6, d12: n = 7) and Trf hpx/hpx (d6: n = 7, d12: n = 4) mice at 6 and 12 days of age. c, Comparison of absolute numbers of DETC (CD3hi TCRβ-), CD8T (CD3+CD8+), and MAIT cells (MR1-5−OP-RU-tet+TCRβ+) in the skin of Trf+/hpx (d6: n = 6, d12: n = 8 for DETC, d12: n = 6 for CD8T, d12: n = 3 for MAIT) and Trf hpx/hpx (d6: n = 3, d12: n = 7 for DETC, d12: n = 4 for CD8T, d12: n = 3 for MAIT) mice at 6 or 12 days of age. d, Histograms of expression of PLZF and T-bet in skin iNKT cells of BALB/c mice at 1–3 weeks of age. e, FC analysis of expression of CCR6 versus ROR𝞬t in skin iNKT cells of Trf+/hpx and Trf hpx/hpx mice at 12 days of age. f, Schematic of Trf administration into Trf hpx/hpx mice. g, Images of Trf +/hpx and Trf-treated Trf hpx/hpx mice at 12 days of age. h, Comparison of absolute numbers of iNKT (CD45+CD1d-tet+) and Treg (CD45+FOXP3+) cells in the skin of Trf+/hpx (n = 5) and Trf hpx/hpx (n = 5) mice after Trf treatment. i,j, Comparison of frequency of iNKT (CD3+CD1d-tet+) and Treg (CD3+FOXP3+) cells in the sLNs (i) and spleens (j) of Trf+/hpx (n = 5) and Trf hpx/hpx (n = 5) mice after Trf treatment. Short lines indicate means. Bars represent mean+s.e.m. One dot represents one mouse. Significance was determined by unpaired two-sided Student’s t-test (a, b, c, i, and j) or unpaired two-sided Mann-Whitney test (h). NS, not significant.
