Extended Data Fig. 9: iNKT cells regulate skin cell homeostasis through Trf-mediated regulation of iron homeostasis.
a, Histograms of expression of PLZF, T-bet, and ROR𝞬t in donor iNKT cells from the skin of 12-day-old Trf hpx/hpx mice that received transfer of WT iNKT cells. b, Images of Trf +/hpx and Trf hpx/hpx mice that received transfer of WT iNKT cells or PBS. c, Comparison of MFI of Ki67 expression in CD45−CD34+ hair follicle stem cells from the skin of Trf +/hpx mice (n = 6) and Trf hpx/hpx mice that received PBS (n = 4) or WT iNKT cells (n = 3). Data are representative of 3 independent experiments. d, Histogram (left) and MFI (right) of CD71 expression in CD45−CD49f+ hair follicle progenitor cells from the skin of Trf +/hpx (n = 13) and Trf hpx/hpx mice that received PBS (n = 8) or iNKT cells (n = 6). Data are representative of 6 independent experiments. e, Histogram (left) and MFI quantification (right) of intracellular ferrous (Fe2+) in skin CD45+ cells of Trf +/hpx (n = 7) and Trf hpx/hpx mice that received PBS (n = 4) or iNKT cells (n = 3). Data are representative of 3 independent experiments. Bars represent mean+s.e.m. One dot represents one mouse. Significance was determined by one-way ANOVA with Dunnett post test (c and d) or Brown-Forsythe and Welch ANOVA with Welch’s correction (e). NS, not significant.
