Extended Data Fig. 1: iNKT cells are highly enriched in the skin of infant and young mice independently of microbiota exposure. | Nature Immunology

Extended Data Fig. 1: iNKT cells are highly enriched in the skin of infant and young mice independently of microbiota exposure.

From: Developmentally programmed early-age skin localization of iNKT cells supports local tissue development and homeostasis

Extended Data Fig. 1

a, Representative FC plots of epidermal γδ T (CD3hiTCRβ-), dermal γδ T (CD3medTCRβ-), and αβ T (CD3medTCRβ+) cells from the skin of WT B6 mice at 3 weeks of age (top) and average percentages and numbers of epidermal and dermal γδ T cells in the skin of mice over time (bottom) (9d: n = 3, 12d: n = 16, 18d: n = 49, 25d: n = 34, 5wk: n = 32; 9–14wk: n = 13). b, Representative FC plots of iNKT (CD1d-tet+), Treg (FOXP3+) and CD8 αβ T cells (CD8+) of the gated skin αβ T cells (CD45+TCRβ+) from WT B6 mice at different ages. c, Representative FC plots of MAIT cells (MR1-5-OP-RU-tet+) of gated skin T cells (CD45+CD3med) at different ages (top). FC plots of MR1-Ac-6-FP tetramer staining are included as negative controls (bottom). d, FC analysis of expression of CD86 and CD8b in skin αβ T cells at 3 weeks of age. Boxplots indicate the value of median (horizontal line), 25th to 75th percentile (box), and min-max range (whisker) of the values. One dot represents one mouse.

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