Extended Data Fig. 8: Diet-derived oxysterols shape spatial localization of IgA+ plasma cells in lamina propria.
From: An epithelial cell-derived metabolite tunes immunoglobulin A secretion by gut-resident plasma cells
a, Representative immunofluorescence of duodenum sections from mice treated for 24 hrs with NF, HCF and VF. b, Quantification of PC frequency at the indicated distance from lymphatics (LYVE1+) in duodenum of mice treated as in (a). Each dot represents the average of PCs distance measured from n = 6 mice per each treatment. c, Quantification of PCs distance from lymphatics in mice treated with the indicated food changes. Each dot represents a single PC. d, Representative IgA ELISpot and compiled data from duodenum of untreated or fasted mice for 12 hours. e, Frequency of IgA+CD98hi cells analyzed by flow cytometry in LP of mice fasted for 12hrs, or untreated. The results were pooled from three experiments (a,b and c)(n = 7 mice per group and n = 50–145 cells (c)) and two independent experiments (d, e)(n = 3–5 mice per group). Statistics were calculated with two-way ANOVA with Bonferroni correction (*p < 0.05, **p < 0.01,***p < 0.001, ****p < 0.0001) in (b and c) and two-sided unpaired Student’s t-test (**P < 0.01,****P < 0.0001) in (d and e). Exact P values are provided in Source data. The error bars represent the mean ± s.e.m.
