Extended Data Fig. 1: 7α,25-HC production is mainly restricted to the duodenum.
From: An epithelial cell-derived metabolite tunes immunoglobulin A secretion by gut-resident plasma cells
a, Ch25h and Cyp7b1 mRNA expression (A.U.) in duodenum and ileum of C57BL/6 mice. b, 7α,25-HC reduction in bile acids precursor (4-cholestern-7 α,25-ol-3-one) by HSD3B7. c, Hsd3b7 mRNA expression (A.U.) in duodenum and ileum of C57BL/6 mice. d, Relative migration of GPR183+ cells with tissue lipid extracts from duodenum and ileum. e, mRNA quantification of Ch25h, Cyp7b1, Hsd3b7 in IECs and LP of C57BL/6 mice. f, Representative flow cytometry plot and number of GPR183+ cells migrating upon exposure to lipid extracts from duodenal and ileal IECs and lamina propria used in Fig. 1c, d. g, Schematic depiction of in vitro 25-HC quantification strategy. The results were pooled from three independent experiments (a,c,d and e)(n = 5 mice per group). Statistics were measured as two-sided unpaired Student’s t-test (*p < 0.05,**p < 0.01,***p < 0.001) in (a,c,d and e) with Bonferroni correction. Exact P values and adjustments are provided in Source data.The error bars represent the mean ± s.e.m.
