Extended Data Fig. 4: Immunophenotypic analysis of TNF-signaling components in hematopoietic precursors and during early T cell development.
a, Flow cytometric dot plots showing expression of TNFR2 and tmTNF (n = 4) on the 5 cellular subsets of ex vivo human immature thymocytes as shown in (Fig. 5a, b). b, ATOs were assembled using human HSPCs derived from fetal liver (CD34hiCD45+; n = 2), cord blood (lin−CD34+CD38−; n = 2) or adult buffy coats (lin−CD34+; n = 4), and harvested for flow cytometry analyses at day 2, 4, 7 and 10 post culture. For each ontogenetic stage, expression of TNFR1 across different time points is compared to the FMO control and is shown in representative offsetting histograms. c,d, Flow cytometric analysis illustrates the TNFR2 expression profiles of human HSPCs from fetal liver, cord blood and adult buffy coat (n = 2 donors for each) (c). Median fluorescent intensity of TNFR2 for HSPCs from different sources (d). ATOs from each ontogenetic stage were assembled and analyzed independently (b). Data are representatives (a,c) and presented as mean (d) of one experiment; n, biological replicates (d).
