Extended Data Fig. 5: Generation of a MS5 cell line that co-expresses human DLL4 and tmTNF (MS5-DLL4/tmTNF). | Nature Immunology

Extended Data Fig. 5: Generation of a MS5 cell line that co-expresses human DLL4 and tmTNF (MS5-DLL4/tmTNF).

From: Intrathymic dendritic cell-biased precursors promote human T cell lineage specification through IRF8-driven transmembrane TNF

Extended Data Fig. 5

a, 6 weeks after sorting, based on co-expression of GFP (reporter for DLL4-encoding construct) and BFP (reporter for TNF-encoding construct), expression of human tmTNF and DLL4 on the transduced MS5 cells was determined by flow cytometry. The MS5 parental cell line served as a negative control. Previously generated MS5-DLL4 cell line served as a positive control for DLL4 expression. b, Expression of reporters, DLL4 and tmTNF on MS5-DLL4/tmTNF cells over time after sorting. Data are representative of one experiment.

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