Extended Data Fig. 2: Bcl6 expression by CD19+ B cells is required for optimal infiltration by B cell subsets in the Mtb-infected lung. | Nature Immunology

Extended Data Fig. 2: Bcl6 expression by CD19+ B cells is required for optimal infiltration by B cell subsets in the Mtb-infected lung.

From: Antigen-specific B cells direct T follicular-like helper cells into lymphoid follicles to mediate Mycobacterium tuberculosis control

Extended Data Fig. 2

scRNA-seq of cells isolated from the lungs of C57BL/6 mice, either uninfected (n = 2 mice), or Mtb-infected at 50 dpi (n = 3 mice) or 100 dpi (n = 3 mice). (a) Swarm plot showing expression of Bcl6, a downstream target of Irf4, in CD4+, CD8+ T and B cells. Bcl6fl/fl, CD4creBcl6fl/fl, and CD19creBcl6fl/fl mice (n = 5–9 mice/group) were infected with Mtb HN878, euthanized at 50 and 100 dpi, and lungs analyzed by flow cytometry for (b) MHC-IIhi AMs, (c) neutrophils, (d) total B cells, (e) GC B cells, and (f) FO B cells. (g) IgG2a and IgG2b antibody specific against Mtb antigen CFP-10 in peripheral blood serum (diluted 1:30) collected at 50 dpi. (h) Tfh-like cells in the lungs of Bcl6fl/fl vs CD19creBcl6fl/fl mice. Data represent mean + SD, analysis was performed using Kruskal-Wallis ANOVA with Dunn’s multiple comparison test (b), one-way ANOVA with Tukey’s multiple comparison test (c to g), and two-sided unpaired t-test (h). *, p ≤ 0.05; **, p ≤ 0.005; ***, p ≤ 0.0005.

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