Extended Data Fig. 3: Blimp1 deficiency in B cells impairs antibody production in mice. | Nature Immunology

Extended Data Fig. 3: Blimp1 deficiency in B cells impairs antibody production in mice.

From: Antigen-specific B cells direct T follicular-like helper cells into lymphoid follicles to mediate Mycobacterium tuberculosis control

Extended Data Fig. 3

iABfl/fl, and CD19creiABfl/fl mice (n = 6–8 mice/group) were infected with Mtb HN878, euthanized at 50 and 100 dpi, and lungs analyzed by flow cytometry for (a) Tfh-like cells. Blimp1fl/fl, and CD19creBlimp1fl/fl mice (n = 6–8 mice/group) were infected with Mtb HN878, euthanized at 50 and 100 dpi. Lungs analyzed by flow cytometry for (b) Tfh-like cells. (c) Immunoglobulin specificity against Mtb antigen CFP-10 in peripheral blood serum (diluted 1:30) collected at 50 dpi were detected by ELISA. Il10fl/fl, and CD19creIl10fl/fl mice (n = 6–8 mice/group) were infected with Mtb HN878 and euthanized at 50 and 100 dpi. (d) Tfh-like cells were enumerated by flow cytometry. Data represent mean + SD and statistical analysis was performed with two-sided unpaired t-test. *, p ≤ 0.05; **, p ≤ 0.005; ***, p ≤ 0.0005.

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