Extended Data Fig. 4: IghelMD4 mice show reduced Mtb-specific antibody production. | Nature Immunology

Extended Data Fig. 4: IghelMD4 mice show reduced Mtb-specific antibody production.

From: Antigen-specific B cells direct T follicular-like helper cells into lymphoid follicles to mediate Mycobacterium tuberculosis control

Extended Data Fig. 4

B6 and IghelMD4 mice (n = 5–10 mice/group) were infected with Mtb HN878, euthanized at 50, 75 and 100 dpi and organs were collected and processed. (a) CD44hiCD4+ T cells, (b) IFNγ-producing CD4+ T cells, (c) TNFα-producing CD4+ T cells, (d) total B cells, (e) GC B cells, and (f) FO B cells were determined by flow cytometry. (g) Immunoglobulin specificity against Mtb antigen CFP-10 in peripheral blood serum (diluted 1:15) collected at 100 dpi. Data is mean + SD, analysis was performed using two-sided unpaired t-test (a to f), and two-sided Mann-Whitney U-test (g). *, p ≤ 0.05; **, p ≤ 0.005.

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