Extended Data Fig. 1: Irf4 expression by CD19+ B cells is required for accumulation of FO B cells in the lung. | Nature Immunology

Extended Data Fig. 1: Irf4 expression by CD19+ B cells is required for accumulation of FO B cells in the lung.

From: Antigen-specific B cells direct T follicular-like helper cells into lymphoid follicles to mediate Mycobacterium tuberculosis control

Extended Data Fig. 1

Irf4fl/fl, CD4creIrf4fl/fl, and CD19creIrf4fl/fl mice (n = 5–10 mice/group) were infected with Mtb HN878 and euthanized at 25 and 50 dpi. (a) MHC-IIhi AMs, (b) neutrophils, (c) total B cells, (d) GC B cells, and (e) FO B cells were determined by flow cytometry. Sera collected from mice (50 dpi; dilution shown 1:30) was analyzed via ELISA for levels of (f) IgG2a and IgG2b specific for Mtb antigen CFP-10. Data represent mean + SD, analysis was performed using one-way ANOVA with Tukey’s multiple comparison test (a, c, d, f), and Kruskal-Wallis ANOVA with Dunn’s multiple comparison test (b, e). *, p ≤ 0.05; **, p ≤ 0.005; ***, p ≤ 0.0005.

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