Extended Data Fig. 5: scWB scRNA-seq SRS gene expression analysis and SRS neutrophil functional interrogation.

a, b, Volcano plots of DGE analysis between SRS groups for pseudobulked (a) neutrophil states and (b) mononuclear cells (red denoting genes with fold change > 1.2 and FDR < 0.1) (positive fold change denoting upregulation in SRS1). c, Correlation (Spearman’s Rho 95% confidence interval) of SRS1 sample median neutrophil IL1R2 gene expression as measured by scRNA-seq with median neutrophil IL1R2 cell surface protein expression as measured by flow cytometry (n = 14). d, Proliferative fraction of anti-CD3/28 bead-stimulated CD4+ T cells after co-culture with neutrophils at a 4 neutrophil:1 T cell ratio, compared to positive controls of CD4+ T cells cultured with anti-CD3/28 beads alone (n = 6 SRS1, n = 13 non-SRS1). Boxplots denote minimum and maximum with whiskers and bottom quartile, median and upper quartile with the box. e, Neutrophil phagocytosis with and without GM-CSF stimulation (n = 15). Boxplots denote minimum and maximum with whiskers and bottom quartile, median and upper quartile with the box. P-values for neutrophil functional assays were calculated with two-sided Wilcoxon rank-sum tests. SRS, sepsis response signature; FDR, false discovery rate; ns, not significant. Neu, neutrophils.