Extended Data Fig. 4: B cell subsets at homeostasis.
From: Distinct metabolic requirements regulate B cell activation and germinal center responses
a, Representative flow plot of splenic transitional T1 (B220+CD93+CD23−IgMhi), T2 (B220+CD93+CD23+IgMhi) and T3 (B220+CD93+CD23+IgMlow) B cells. Live singlets from the spleen were first gated for B220+CD93+ cells and then gated for surface expression of IgM and CD23 as indicated. b, Quantification of T1, T2, and T3 B cells in the spleens of mice of indicated genotypes. c, Representative flow plots of B1a (B220lowCD19+CD5+) B cells in the peritoneum of mice. Live singlets from the peritoneal cavity were first gated for B220lowCD19+cells and then gated for surface expression of CD52. d, Quantification of B1a cells in the peritoneum of mice of the indicated genotypes. e, Representative flow plots of marginal zone (MZ) B cells and follicular zone (FO) B cell subsets in the spleen of mice of the indicated genotypes. Live singlets from the spleen were first gated for mature B220+CD93−B cells and then analyzed for surface expression of CD21 and CD23. f,g, Frequency and absolute number of FO B cells. h, i, Frequency and absolute number of MZ B cells. j, Frequency of CD4+ and CD8+ T cells (among live singlets) in peripheral blood and k, in the spleen of mice of the indicated genotypes. Each datapoint represents a single mouse. Bars represent mean. *p ≤ 0.05, **p ≤ 0.01, p-values were calculated using unpaired, two-tailed t-test. For panels b, d, f–k, n = 5 of each genotype and data is representative of two independent experiments.
