Extended Data Fig. 5: Confirmation of Ldha deletion in CD138+ plasmablasts, marginal zone (MZ) and follicular (FO) B cells; IgM ELISA of culture supernatants of ex vivo FO and MZ B cells.
From: Distinct metabolic requirements regulate B cell activation and germinal center responses
a, Gating strategy for the analysis of B220lo CD138+ cells in the spleens of mice challenged with LPS. b, Representative PCR showing the genomic deletion of the floxed Ldha exon in CD138+ plasmablasts sorted from the spleens of Ldhafl/flCd23Cre and control mice at d5 after LPS challenge. Tail DNA was used as a control to detect both the null and floxed alleles. Image of ethidium bromide-stained gel shown is representative of three independent experiments. c, Purified FO or MZ B cells of the indicated genotypes (n = 3) were cultured ex vivo for 72 h with LPS + IL4 or LPS respectively and IgM concentration in the culture supernatants was determined by ELISA. Bars represent mean ± s.e.m. d, Representative PCR depicting genomic deletion of Ldha from sorted FO and MZ B cells cultured with LPS. Image of ethidium bromide-stained gel shown is representative of three independent experiments.
