Extended Data Fig. 6: Gating strategy for analysis of GC B cells following NP-CGG immunization, quantification of GC density and gating strategy for sorting of GL7+ and GL7− B cells.
From: Distinct metabolic requirements regulate B cell activation and germinal center responses
a, Gating strategy for analysis of GC B cells and associated populations in the spleens of NP-CGG immunized mice. b, Mice were immunized intraperitoneally with NP-CGG, boosted at d10 and the GC density in the spleen sections from Ldha+/+ Cd23Cre (grey bar) and Ldhafl/fl Cd23Cre (red bar) mice was quantified at d14 post-immunization. Each data point represents one independent experiment in which data from the spleen sections of 3 mice per genotype were pooled. Data presented is mean ± s.e.m. c, Detection of pre-GC B cells. Wild type mice were immunized intraperitoneally with NP-CGG or with PBS and the frequency of GL7+ cells quantified at d4 post-immunization. d, Gating strategy for the sorting of GL7+ and GL7− cells from the spleens of NP-CGG immunized mice at d4 post-immunization. Sorted GL7+ and GL7− cells were mixed 3:1 and subjected to scRNA sequencing analysis. Flow cytometry of the post-sort cells is shown.
