Extended Data Fig. 1: CD44^s-hiCD49d^lo CD8⁺ T cells are exclusively found in AA SDLNs, and CD44^s-hiCD49d^lo CD8⁺ T cells from skin exhibit higher expression of genes relevant for cell motility and inflammatory response compared to CD44^s-hiCD49d^lo CD8⁺ T cells from SDLNs.

a, The proportion of CD44^highCD49d^lo CD8⁺ T cells in SDLN CD8⁺ T cells (n = 16, naive; n = 29, AA). b, CD44^s-hiCD49d^lo CD8⁺ T cells were nearly absent in the spleens, liver, and skin non-draining mesenteric lymph nodes of alopecic mice (n = 5, liver; n = 4, others). c, Heatmap of differentially expressed genes (DEGs) in CD44^s-hiCD49d^lo CD8⁺ T cells from SDLNs relative naive T_N cells, naive T_VM cells, and AA T_VM cells (pairwise comparison). Numbers in overlapping regions indicate gene transcripts shared by the overlapping DEG, whereas numbers in non-overlapping regions indicate unique DEGs to each cell subset. d, e, Heatmap of DEGs from skin CD44^s-hiCD49d^lo CD8⁺ T cells compared to SDLN CD44^s-hiCD49d^lo CD8⁺ T cells (d) and enriched GO biological process gene sets (padj < 0.05) (e). Data were acquired from AtAA mice. mLN, mesenteric lymph node. Data are presented as mean values ± SD. A Mann-Whitney test was performed for comparisons between two groups. All tests were two sided. *P < 0.05, ****P < 0.0001.

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