Extended Data Fig. 4: Conventional memory CD8⁺ T cells primed by skin infection do not contribute to the induction of CD44^s-hiCD49d^lo CD8⁺ T cells.

a, Experimental scheme for testing whether skin infection could contribute to the induction of AA. After 4 weeks of vaccinia virus (VV) infection by skin scarification, VV-infected mice (VV-AT) or naïve mice (Control-AT) were induced AA by adoptive transfer method. b,c, Representative flow cytometry plots (b) and the proportion of true memory (T_TM) T cells in CD8⁺ T cells 7 days post-VV infection (n = 3) (c). d, Disease free ratio between the VV-AT (n = 8), the control-AT (n = 10), and the historical control group (n = 74). e, Representative flow cytometry plots of CD8⁺ T cells after 12 weeks of adoptive transfer. f, Frequency of each cell populations in CD8⁺ T cells (n = 7, Control-AT; n = 5, VV-AT). g, gMFI of the NKG2D level in each cell population (n = 7, Control-AT; n = 5, VV-AT). Data are presented as mean values ± SD. A Log-rank (Mantel-Cox) test was used for comparison of survival curves. A Mann-Whitney test was performed for comparisons between two groups. All tests were two sided.

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