Extended Data Fig. 5: Single-cell transcriptome analysis of ThPOK-/- and WT BM populations.
From: ThPOK is a critical multifaceted regulator of myeloid lineage development
a) UMAP plot of curated cKit+ progenitor scRNA-Seq cell populations that serves as the reference for cellHarmony alignment analyses in these studies (see Methods), b) Flow cytometry selection of c-Kit+ BM C-GMP progenitors (gated cells denoted in blue). c) Unsupervised clustering UMAP plot of ~26,000 combined C-GMP CITE-Seq captured mRNA profiles (WT and ThPOK-/-) following analysis with the software ICGS2. Indicated cell-population labels are those automatically assigned by ICGS2 (AltAnalyze BioMarker database). d) UMAP plot of all WT and ThPOK-/- cells from panel c aligned to the reference described in panel a. e) UMAP plot from panel d showing the expression levels of ThPOK mRNA in the wild-type CITE-Seq populations. f) Cell-population percentage of distinct BM progenitor populations detected by CITE-Seq from WT and ThPOK-/- mice. g, h) Heatmap of relative normalized (TotalVI) CITE-Seq antibody derived-tag (ADT) intensities for cellHarmony cKit+ aligned cell populations. Panels (g) and (h) displays cells from WT and ThPOK-/- BM progenitors, respectively. i-p) Gene Ontology enrichment analyses from the software GO-Elite, for each of the indicated cell population differential expression analysis comparisons (all ThPOK-/- vs. WT).
