Fig. 5: Multiple iPSC-line-derived iMGLs also exhibit transcriptional diversity and DAM induction. | Nature Immunology

Fig. 5: Multiple iPSC-line-derived iMGLs also exhibit transcriptional diversity and DAM induction.

From: Exposure of iPSC-derived human microglia to brain substrates enables the generation and manipulation of diverse transcriptional states in vitro

Fig. 5

a, UMAP projection of integrated H1 iMGL and iPSC iMGL profiles; cells are colored by identity of H1_iPSC_iMGL joint clusters. b, Proportion of cells per H1_iPSC_iMGL joint cluster for each dataset (either iMGL H1 replicates or three separate iPSC lines differentiated into iMGLs). c, UMAP projection as in a. Green, iMGL_2; magenta, iMGL_8. d, UMAP projection (as in a) of the shared metagene common to both datasets in cluster 6. Right, top constituent genes of this shared factor. e, Violin plots showing expression of GPNMB and LPL across joint clusters in a. Boxes show the first and third quartiles of the data, with a line marking the median. Whiskers mark values closest to 1.5 times the interquartile range; no outliers are plotted. f, Percentages of nontreated (control) and AN-treated cells in joint cluster 6, from iPSC-derived iMGL only (two-tailed t-test, P = 0.00471; n = 3; mean and s.e.m. are shown). In c and d, the black arrow highlights joint cluster 6.

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