Extended Data Fig. 6: Effects of in vivo C2 and C4 supplementation.
From: Short-term dietary changes can result in mucosal and systemic immune depression
(a, b) Representative dot plots (left) and frequencies (right) of IFN-γ-secreting CD4+CD44hi memory T after in vitro PMA/Ionomycin re-stimulation isolated from spleens of mice switched to FD or kept on RD, day 8 (A) or 22 (B) after priming. (c) Body weight normalized to day 0 of RD- and FD-fed mice (+/− C2/C4 supplementation). (d) Food intake (kcal/day) of RD- and FD-fed mice (+/− C2/C4 supplementation). (e) Water intake (mL/day) of RD- and FD-fed mice (+/− C2/C4 supplementation). (f) PCA of stools isolated from RD- and FD-fed mice (+/− C2/C4 supplementation). (g) Relative abundance of bacterial species in stools isolated from RD- and FD-fed mice (+/− C2/C4 supplementation). (h) LDA score showing differentially abundant bacteria in FD vs FD + C2/C4 supplementation. (i) Frequencies of PP TH17 cells among Tconv cells isolated from mice fed RD or switched to FD for 3 days with or without C2/C4 supplementation in their drinking water (FD + C2/C4). Data in (A-B) are a pool of 2 experiments (n = 9 or 8). Data in (C-E) are from a pool of 2 experiments (weight gain, n = 9 or 10; food and water, n = 4 or 5, measured per cage). Data in (F-H) are from one experiment (n = 5 or 6). Data in (I) are a pool of 2 experiments (n = 6, 5 or 8). Data are shown as mean ± SEM. P.values have been determined by two-tailed non-parametric Mann-Whitney (A-B) or Kruskal-Wallis test with Dunn’s multiple comparisons test (I).
