Fig. 2: Short-term consumption of FD affects the intestinal CD4+ T cell compartment.
From: Short-term dietary changes can result in mucosal and systemic immune depression
a, Dietary intervention schematic. b, Volcano plot showing DEGs (Padj ≤ 0.10, |fold change| ≥ 1.3) in FACS-sorted CD4+Foxp3− T cells isolated from PPs of RD-fed and FD-fed mice. Two to three biological replicates per group were sequenced. c, Left, representative dot plots of exTH17 (YFP+Katushka−) and TH17 (YFP+Katushka+) cells gated on CD3+CD4+Foxp3− viable conventional T (Tconv) cells in PPs of RD-fed and FD-fed mice. Right, frequencies of exTH17, TH17 and IL-10-secreting TH17 cells among CD3+CD4+Foxp3− Tconv cells in PPs of RD-fed and FD-fed mice. d, Heatmap showing expression level of Reg3b, Reg3g, Muc1 and Muc2 normalized on Tbp and shown as relative to RD. e, Dietary intervention and S. Typhimurium infection model. f, CFUs of S. Typhimurium in SI luminal content, SI tissue and liver in IL-17A/IL-17F double-knockout and littermate control mice upon switch to FD. Data in c are a pool of two experiments (exTH17 and TH17, n = 9 or 7; IL-10+ TH17, n = 8 or 5, representative of four experiments). Data in d are a pool of two experiments (n = 10 or 9). Data in f are a pool of five experiments (SI content, n = 23, 16, 18 or 19; SI tissue, n = 16, 19, 25 or 19; liver, n = 21, 12, 27 or 12). Data are shown as mean ± s.e.m. or median (f). P values were determined using two-tailed Wald test (b), two-tailed nonparametric Mann–Whitney U-test (c,d) or Kruskal–Wallis test with Dunn’s multiple comparison test (f). P values in d: a, 0.0115; b, 0.0005; c, <0.0001; d, 0.0185.
